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In Vitro Mutagenesis: Methods and Protocols

In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performingin vitromutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis.In Vitro Mutagenesis: Methods and Protocolsguides readers through methods for gene and genome editing...

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Statistical Methods in Bioinformatics

Advances in computers and biotechnology have had a profound impact on biomedical research, and as a result complex data sets can now be generated to address extremely complex biological questions. Correspondingly, advances in the statistical methods necessary to analyze such data are following closely behind the advances in data generation methods. The statistical methods required by bioinformatics present many new and difficult prob...

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Plant Ecology

This textbook covers Plant Ecology from the molecular to the global level. It covers the following areas in unprecedented breadth and depth: - Molecular ecophysiology (stress physiology: light, temperature, oxygen deficiency, drought, salt, heavy metals, xenobiotica and biotic stress factors) - Autecology (whole plant ecology: thermal balance, water, nutrient, carbon relations) - Ecosystem ecology (plants as part of ecosystems, eleme...

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MicroRNA Profiling: Methods and Protocols

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocolsdiscuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived pr...

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Mammary Gland Development: Methods and Protocols

This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge be...

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Cohesin and Condensin: Methods and Protocols

The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fund...

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Cell Cycle Synchronization: Methods and Protocols, 2nd edition

This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters inCell Cycle Synchronization: Methods and Protocols, Second Editioncover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchr...

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Planar Cell Polarity During Development

This new volume of Current Topics in Developmental Biology covers the area of Planar Cell Polarity with contributions from an international board of authors. The 12 chapters provide a comprehensive set of reviews covering such topics as PCP in Zebra fish, the role of Dishevelled in PCP regulation, and PCP in axon pathfinding. Covers the area of planar cell polarity International board of authors 12 chapters provide a comprehensive se...

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Staying with the Trouble: Making Kin in the Chthulucene

In the midst of spiraling ecological devastation, multispecies feminist theorist Donna J. Haraway offers provocative new ways to reconfigure our relations to the earth and all its inhabitants. She eschews referring to our current epoch as the Anthropocene, preferring to conceptualize it as what she calls the Chthulucene, as it more aptly and fully describes our epoch as one in which the human and nonhuman are inextricably linked in t...