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Computational Protein Design

The aim this volume is to present the methods, challenges, software, and applications of this widespread and yet still evolving and maturing field. Computational Protein Design, the first book with this title, guides readers through computational protein design approaches, software and tailored solutions to specific case-study targets. Written in the highly successful Methods in Molecular Biology series format, chapters include intro...

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T-Helper Cells: Methods and Protocols

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell functi...

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Plant Pathology Concepts and Laboratory Exercises, Third Edition

Continuing in the tradition of its predecessors, this new edition combines an informal, easy to read style with a thorough introduction to concepts and terminology of plant pathology. After reviewing fundamental concepts, the book discusses groups of plant pathogens and molecular tools for studying them, pathogen interactions, epidemiology and disease control, and special topics in plant pathology. The book details various disease-ca...

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Spliceosomal Pre-Mrna Splicing

Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapte...

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Understanding Mammalian Locomotion: Concepts and Applications

Understanding Mammalian Locomotion will formally introduce the emerging perspective of collision dynamics in mammalian terrestrial locomotion and explain how it influences the interpretation of form and functional capabilities. The objective is to bring the reader interested in the function and mechanics of mammalian terrestrial locomotion to a sophisticated conceptual understanding of the relevant mechanics and the current debate on...

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The Brain: Big Bangs, Behaviors, and Beliefs

After several million years of jostling for ecological space, only one survivor from a host of hominid species remains standing: us. Human beings are extraordinary creatures, and it is the unprecedented human brain that makes them so. In this delightfully accessible book, the authors present the first full, step-by-step account of the evolution of the brain and nervous system. Tapping the very latest findings in evolutionary biology,...

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In Situ Hybridization Protocols, 4th edition

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,...

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Neural Stem Cells: Methods and Protocols

In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their ...

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Genetically Engineered Salmon: Background and Issues

If approved by the Food and Drug Administration (FDA), Atlantic salmon would be the first genetically engineered (GE) animal to be marketed in the United States for human consumption. Genetic engineering techniques are used by scientists to insert genetic material from one organism into the genome of another organism. Genetically engineered salmon have been modified to grow more quickly and use feed more efficiently. However, some ar...

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Darwin's Plots: Evolutionary Narrative in Darwin, George Eliot and Nineteenth-Century Fiction, 3 edition

Rituals are like containers, gently holding those profound moments and passages in life that we hope to honor, savor, or simply let go of. "They mark significant times, ease us through transitions, and–especially in times of rapid change–bring structure and stability to our lives," explains author Barbara Bizlou, who's known by many as the "Ritual Lady." In her elegant and thorough book, Bizlou offers readers specific ritual suggesti...